Geoff Barton runs the third part of the School of Life Sciences’ Postgrad Computational Training on Multiple Sequence Alignment and Analysis. The afternoon workshop introduced the principles of sequence analysis and its relationship to protein structure and function. It highlighted common methods and tools for protein sequence analysis and multiple sequence alignment, it involved both talks and hands-on exercises. The hands-on examples applied methods that exploit evolutionary information to predict structural features from sequence and to identify functionally important residues by sub-family analysis. The talks considered how best to use computational methods to “make sense” of the structure and function of proteins.